FastQCFastQC Report
Sun 14 Apr 2019
SRR6576330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265942
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4780.17973843920854923No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3390.12747140353911757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAA450.001012069641.781791
GTACATG16850.040.7279471
TACATGG17250.039.783532
ACATGGG16900.039.7655683
GTATCAA7700.036.6268921
CGACGGT801.1560362E-535.2467547
CATGGGG5900.035.0476234
CATGGGA8250.034.1786734
TACGACG851.7508386E-533.173425
ACGACGG902.5881169E-531.330456
TTAATAC953.743908E-529.6814793
GACGGTA953.743908E-529.6814798
ACGGTAT953.743908E-529.6814799
GGGTACC2400.029.3722957
GTAGACT804.7736903E-429.3722954
CGCCGGT650.006148543728.9204167
TAATACT1154.183461E-628.6060644
ATGGGTA2500.028.1974075
GGTACCT2500.028.1974078
ATGGGGA3500.028.1974055
Produced by FastQC (version 0.11.2)