FastQCFastQC Report
Sun 14 Apr 2019
SRR6576329_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576329_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360460
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6940.19253176496698662No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4780.1326083337957055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.11346612661599068No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4080.11318870332353104No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4050.11235643344615215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGC250.005236098556.3919263
GTATCAA11550.043.193931
TACATGG24750.036.4604452
GTACATG25400.036.317941
ACATGGG25000.035.902863
AACGCAG14250.034.9563986
TAGTACC702.1838746E-433.5666244
ATCAACG14550.033.2667163
CATGGGG8900.033.26494
CAACGCA14850.032.9111145
TCAACGC14800.032.3872534
CATGGGT6400.030.1050644
ACGCAGA16450.029.9957057
GGTACCT3150.029.8577088
GTATAGA953.7099948E-529.7253191
CGCAGAG16650.029.6559668
TATCAAC16750.029.181962
ATGGGTA4250.026.5373785
CATGGGA13200.026.3447134
GCAGAGT18800.026.264469
Produced by FastQC (version 0.11.2)