FastQCFastQC Report
Sun 14 Apr 2019
SRR6576329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360460
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8170.2266548299395217No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4820.13371802696554402No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4750.13177606391832658No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4410.12234367197469899No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.11429839649336959No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3740.10375631137990345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA401.035005E-982.228273
GTACCGT250.005238606656.3851056
TATCGAG250.005238606656.3851055
CGCTAAA757.4428826E-637.590075
TACATGG22600.037.226162
GTACATG22850.036.818871
GTATCAA10650.035.7469561
ACATGGG23550.035.7145543
CATGGGT5000.035.7105644
GGTACCT2950.033.4487888
ATGGGTA3500.032.220065
TGCCCGC902.5887886E-531.333751
CCGCTAA902.5937035E-531.3250564
GCAAACG804.7740765E-429.375391
CATGGGA13600.028.6762474
ATCAACG12950.028.6642423
AACGCAG13000.028.5539956
TCAACGC13000.028.5539954
GGGTACC3550.027.7954737
CATGGGG7800.027.7106274
Produced by FastQC (version 0.11.2)