FastQCFastQC Report
Sun 14 Apr 2019
SRR6576328_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576328_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393301
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7730.19654158011294148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.11365341049221843No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4300.10933102127886783No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4050.10297456655335227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10800.039.2941631
GTACATG23200.036.5842251
TACATGG23600.035.849832
ACATGGG23500.035.202333
TCAACGC13050.031.335414
AACGCAG13250.031.2171636
ATCAACG13350.030.6312453
ATGGGAG4800.030.356185
CAACGCA13850.030.2041665
CATGGGG8700.028.0938154
GGGTACC3600.027.4149997
CATGGGT5700.027.212334
CATGGGA12700.026.2773324
ATGGGTA3450.025.8857755
TATCAAC16200.025.82272
ACGCAGA15700.025.7436567
TTATACT1101.0162107E-425.6380634
CGCAGAG15600.025.3061528
ATGGGAT4000.024.6766345
GGTACCT3550.023.8295358
Produced by FastQC (version 0.11.2)