FastQCFastQC Report
Sun 14 Apr 2019
SRR6576322_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576322_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252845
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5240.20724159069785836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3460.13684272973560876No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3340.13209673910894026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.12893274535782792No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3030.11983626332337993No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3010.11904526488560184No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2640.10441179378670726No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2560.10124780003559493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG601.5819805E-647.0778661
TATAAGG704.624899E-640.280632
GTACATG18250.037.6622961
TACATGG18300.037.2357332
ACATGGG18350.036.8781783
ACCGTGG702.1742245E-433.587128
CATGGGG6700.032.2645844
ATAGAGT600.00415746931.3293766
ATGGGGA3500.030.8818155
ATGGGAT2500.030.0762025
GTATCAA9600.029.4236661
CATGGGA10400.029.3712924
ATGGGTA2002.5465852E-1128.1964425
TAGGGCT1005.3119205E-528.1964424
GTATAGC856.7357515E-427.6928631
GGGTACC2253.6379788E-1227.1521287
AACGCAG10700.026.3518146
ATGGGAG4000.025.8467375
CATGGGT3500.025.5110654
GACCGCG1301.08452E-525.3044977
Produced by FastQC (version 0.11.2)