FastQCFastQC Report
Sun 14 Apr 2019
SRR6576321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277023
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6020.21731047602545636No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3460.12489937658605964No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3430.1238164340145042No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.11695779772798649No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3180.11479191258487562No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3160.11406995087050534No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3050.11009916144146877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8950.036.2455671
TACATGG18150.034.4511572
ACATGGG18250.034.2500153
TATATGG550.002709455634.1921272
TATATGC550.002709455634.1921272
GTACATG18450.033.890981
ATGGGAG4650.033.352855
ATATACT753.2703183E-431.3314674
CATGGGT3700.030.4846694
AACGCAG11450.028.7319136
CATGGGA10000.028.1983224
CAACGCA11750.027.9983335
ATCAACG11800.027.4814153
TATATAC1205.824917E-627.4150353
TCAACGC11900.027.2504794
AAGCGAC350.00883470726.85554570-71
TTCGACA350.00883470726.85554512-13
CATGGGG6350.026.6440834
GGGTACC2500.026.3184347
ACGCAGA13000.025.3061877
Produced by FastQC (version 0.11.2)