FastQCFastQC Report
Sun 14 Apr 2019
SRR6576320_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576320_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701857
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17590.2506208529657751No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11760.1675554991971299No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11550.16456343671146687No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10810.15401997842865428No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT9390.1337879368589328No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG8980.1279462910535907No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA8910.126948936891703No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG8820.12566662439784743No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8270.11783027026872996No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG8190.11669043694085832No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG7990.11384085362117924No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC7910.11270102029330761No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT7680.10942399947567666No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC7360.10486466616419016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17100.047.9031681
GTACATG40350.038.7351881
ACATGGG41150.037.1150863
TACATGG41800.037.100062
TCAACGC22800.036.0695044
AACGCAG23450.035.6709066
ATCAACG23100.035.3976363
CAACGCA23750.034.824595
CATGGGT11750.032.7954034
ATGGGTA7550.031.1214645
ACGCAGA27200.030.580277
CGCAGAG28700.029.1644388
CATGGGG18750.028.3213624
ATGGGAG8300.028.3092845
TATCAAC30200.027.853712
GGTACCT7450.027.7723058
CATGGGA17550.027.5801774
GGGTACC7900.027.363257
CTATTAA2104.7293724E-1126.901291
GCAGAGT31050.026.5028119
Produced by FastQC (version 0.11.2)