FastQCFastQC Report
Sun 14 Apr 2019
SRR6576320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701857
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19540.2784042903326461No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA13780.19633629072588862No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12410.1768166449860869No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA11550.16456343671146687No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10360.14760841595937635No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG8750.12466927023595975No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA8740.1245267910699758No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT8700.12395687440603997No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC8310.11840018693266578No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG7790.11099127030150018No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC7720.10999391613961249No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG7600.10828416614780505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.1068593744879655No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT7450.10614697865804572No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG7330.10443722866623828No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG7110.1013026870145913No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7060.10059029118467153No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC7060.10059029118467153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT450.00101470341.769267
GTATCAA17000.040.3651051
GTACATG42250.035.1530081
ACATGGG41250.034.744433
TACATGG44300.033.311722
ATATAAC753.2781693E-431.3269443
TGCGTAT600.004165492531.32471594
CATGGGG18350.030.7294274
AACGCAG22500.030.2827136
TAATACG1252.3651955E-730.0738683
TCAACGC22900.029.548564
ATCAACG23150.029.4324443
CAACGCA23000.029.4200865
CGGTATA650.00615440428.92132
ATACGTC1503.700734E-828.194255
CATGGGT11250.027.567714
GTATAGG2156.548362E-1126.2328171
ATGGGTA8100.026.1057875
ACGCAGA26200.025.6474427
CATGGGA18000.025.5836724
Produced by FastQC (version 0.11.2)