FastQCFastQC Report
Sun 14 Apr 2019
SRR6576319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774151
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20330.2626102659558665No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA13730.177355580500445No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11740.15165000109797702No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10580.1366658442603575No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10400.13434071647520962No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA9350.1207774710618471No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT8930.1153521728965021No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC8760.11315621887719579No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG8480.10953935343363246No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG8160.10540579292670293No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT7940.10256397007818888No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG7850.10140140618561495No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG7800.1007555373564072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.10023884229304102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17750.049.253481
CTATATT902.5102054E-1046.999614
GTACATG42650.039.8943941
TACATGG43950.038.9282462
ACATGGG43400.038.877563
ATGGGTA8350.037.7122655
ATCAACG23650.035.3739133
CATGGGT11600.035.249714
GGGTACC7650.035.0193187
AACGCAG23900.034.8072436
TCAACGC24200.034.5699654
CAACGCA25150.033.2641375
GGTACCT8250.032.4724588
ATAGACG1201.651224E-731.3330758
TTACGTA600.004160317631.3330754
CATGGGG19650.030.8547064
TGGGTAC8700.030.7928496
ATACGTC1401.8893843E-830.2140395
ACGCAGA27950.029.7636187
CATGGGA18200.029.4393184
Produced by FastQC (version 0.11.2)