FastQCFastQC Report
Sun 14 Apr 2019
SRR6576316_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576316_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437546
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14480.33093663294830716No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8060.18420920314664058No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7620.1741531176150622No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6660.15221256736434569No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6590.15061273557523094No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6160.14078519744209753No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6040.13804262866075795No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5960.13621424947319824No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5800.13255749109807882No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5380.12295850036339036No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5300.12113012117583065No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4900.1119882252380321No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4890.11175967783958717No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4800.10970275125358248No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4770.1090171090582476No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4740.1083314668629127No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4620.10558889808157314No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4610.10536035068312817No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4460.10193213970645372No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4420.10101795011267385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG200.002164032570.483975
CATGGGG12200.036.9751974
ACATGGG29550.034.0294883
GTACATG31050.033.1915361
ATGGGTA6050.032.620685
TACATGG31800.032.21282
GTATCAA14500.031.8055481
TGGGTAC6200.031.0735786
GGGTACC6100.030.8126667
GGTACCT6150.030.5866268
CATGGGT8450.030.0286754
ATGGGGG4200.029.0886215
CCACGAC1005.3243468E-528.193593
GTTACAC700.00883994726.8510383
GTACTTT11450.026.3038481
ATCAACG18050.024.7312183
TAATACC1151.3774291E-424.5161654
TACTTTT12400.024.2525482
AACGCAG17900.024.1509326
TCAACGC18150.024.077174
Produced by FastQC (version 0.11.2)