FastQCFastQC Report
Sun 14 Apr 2019
SRR6576316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437546
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15850.36224762653526715No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8720.19929333144400818No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8680.1983791418502283No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8400.19197981469376935No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7120.16272574769281403No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6970.15929753671613955No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6550.14969854598145108No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5830.13324313329341372No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5790.13232894369963388No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5400.12341559516028029No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5400.12341559516028029No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5160.11793045759760117No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT5150.1177019101991562No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5120.11701626800382132No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4960.1133595096287019No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4920.11244532003492204No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4880.1115311304411422No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4810.10993129865202744No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4750.10856001426135767No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG4690.10718872987068788No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4620.10558889808157314No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT4470.10216068710489869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG12750.037.592874
ACATGGG31000.036.3801963
GTACATG32050.036.0762861
TACATGG31850.035.864212
GTATTAC801.1567026E-535.251371
ATGGGGA7150.031.5464635
GTATCAA15500.031.5367131
CATGGGT9000.029.761024
ATGGGAG4700.027.9946885
GGTACCT5900.027.8760688
AACGCAG17200.027.5936016
CAAACGC700.00882354926.8612582
ATGGGTA6650.026.8520475
TAGTACT700.00883832126.8520474
CAACGCA18050.026.554525
TCAACGC18050.026.554524
TGGGTAC6450.026.2275856
ATCAACG18400.026.0494063
ATAGTAC1101.0182365E-425.63153
ACGCAGA19550.024.2767247
Produced by FastQC (version 0.11.2)