FastQCFastQC Report
Sun 14 Apr 2019
SRR6576315_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576315_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489830
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13730.2803013290325215No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8600.17557111650980953No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8120.16577179837902947No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7170.14637731457852723No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6860.14004858828573177No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6300.12861605046648839No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6260.1277994406222567No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5700.11636690280301329No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5570.11371292080926036No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5490.11207970112079702No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5390.11003817651021783No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5080.10370945021742238No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5050.10309699283424861No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5030.10268868791213279No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4960.10125962068472737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG30950.035.3085141
CATGGGT8500.034.28764
TAGACCG550.002713454734.1870545
ACATGGG31250.034.146033
TACATGG32400.033.6594772
GTATCAA13800.033.1089021
ATGGGTA6000.032.905045
GGTACCT5950.032.3882068
GGGTACC6500.030.367687
TGGGTAC6200.029.5690466
CATGGGG13700.029.1650494
GTCTAAT650.006085082428.98671
GTATAGG1205.7353627E-627.4769761
GTACCTG7200.027.4180689
CGCAACG350.008837596526.8557736-37
TCAACGC16850.026.7815484
CAACGCA17150.026.3130665
AACGCAG17000.025.9922146
ATCAACG17550.025.7133393
ATGGGGC3650.024.46955
Produced by FastQC (version 0.11.2)