FastQCFastQC Report
Sun 14 Apr 2019
SRR6576314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337521
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9160.2713905208861078No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6210.1839885518234421No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5200.15406448783927518No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4600.13628781616551267No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4290.12710320246740203No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4260.1262143688837139No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3800.11258558726716264No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3780.11199303154470389No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3700.10962280865486888No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC3540.10488236287519888No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3510.10399352929151076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA551.6552804E-1068.362025
TAACCTA250.00523026656.4070242
CCGCTAA602.5493136E-854.8320354
CCCGCTA654.7908543E-850.6141853
CCGAACT957.2759576E-1249.5238721
GCAAACG405.642708E-447.0476761
GTATCAA7150.046.7186741
GCTAAAA901.3093086E-841.776796
ATACCGT500.00170052237.5991136
TAGTATG500.00170052237.5991137
GTACTAG651.4029804E-436.190521
CATATAC801.1564167E-535.2491653
CTAGTAC801.1564167E-535.2491653
CGATAAT401.15812945E-535.24916566-67
CAACGCA9550.034.941585
GTACATG17000.034.8706321
CTAAAAA1353.43789E-1034.813997
TCAACGC9650.034.5794944
AACGCAG9700.034.401256
ATCAACG9800.034.0502133
Produced by FastQC (version 0.11.2)