FastQCFastQC Report
Sun 14 Apr 2019
SRR6576313_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576313_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276527
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5000.18081417004487807No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3550.12837806073186345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.12476177733096588No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3260.1178908388692605No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3000.10848850202692685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGA250.005234876656.3917546
TACGTGG450.001013347541.7716676
TACATGG17650.034.082
GTACATG17800.033.8417661
GTATCAA8450.033.4162451
ACATGGG18250.032.9595643
CATGGGG6250.032.3312724
CATGGGT4250.032.06594
CTCTAGT700.00876877826.892121
ATGGGGG2650.026.5998825
TAGTAGT909.5074554E-426.1072924
ATCAACG10400.025.755853
AACGCAG10700.025.472916
ATGGGTA2409.094947E-1225.4546135
TCAACGC10800.024.8019284
TCAGTAC1151.3748815E-424.5181543
CAACGCA10950.024.4621755
GACCGCG1351.4538904E-524.3668067
ATACAAT1351.4538904E-524.3668066
AGGACCG1751.6390914E-724.1678965
Produced by FastQC (version 0.11.2)