FastQCFastQC Report
Sun 14 Apr 2019
SRR6576313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276527
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5370.19419441862819906No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3530.12765480405168392No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2980.10776524534674733No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2830.10234082024540099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG555.2819974E-542.714455
GTACATG16750.037.3149531
GTATAAG906.1733954E-736.5511931
TACATGG16750.036.4732632
GTATCAA9000.036.0290341
CATGGGG6200.035.618344
ACATGGG16800.035.51913
ATGGGTA1950.033.7334635
AACGCAG9350.033.1665156
CAACGCA9750.031.8058365
ATCAACG9850.031.4829353
TCAACGC9950.031.1665254
GGGTACC1901.4551915E-1129.67537
TGTAGTA804.7749365E-429.3714962
ACGCAGA10600.029.2553677
CGCAGAG10750.028.8471538
GTATAAA1853.255991E-1027.9426141
GGTACCT1704.0618033E-927.6387638
CATGGGT4350.027.0033874
TGGGTAC2008.076313E-1025.8422436
Produced by FastQC (version 0.11.2)