FastQCFastQC Report
Sun 14 Apr 2019
SRR6576309_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576309_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220388
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5790.26271847832005374No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3390.15381962720293302No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3320.15064341071201698No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3030.1374847995353649No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.13113236655353286No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2840.12886364048859286No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2810.12750240484962885No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2700.1225112075067608No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2660.12069622665480878No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2530.11479753888596475No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2330.1057226346262047No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2250.10209267292230068No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2240.10163892770931265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7500.045.800411
CATGGGT3050.041.598724
ATGGGTA1750.040.2781265
TACATGG15000.038.219472
GTACATG14950.038.0848241
ACATGGG15250.036.6685073
TCAACGC8950.035.7027744
AACGCAG8700.035.648466
ATCAACG9050.035.3082663
TGGGTAC1900.034.6250576
CAACGCA9350.033.6728025
GTCCTAT851.7343818E-533.2154431
GTATATG902.5638261E-531.3701441
CATGGGA6000.030.5442494
ATGGGGA3850.030.5137335
ACGCAGA10450.029.678627
TATCAAC11000.029.4762672
CATGGGG7850.029.3320564
CGCAGAG10700.029.0049428
GTATACA650.006108371528.9570541
Produced by FastQC (version 0.11.2)