FastQCFastQC Report
Sun 14 Apr 2019
SRR6576308_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576308_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234539
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5920.25241004694315233No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3460.1475234395985316No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3260.13899607314774942No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3130.133453284954741No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3120.1330269166322019No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2700.11511944708555934No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.11170850050524646No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2530.10787118560239449No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2510.10701844895731627No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2450.10446023902208161No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2410.10275476573192517No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2390.10190202908684695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT250.005232785456.3947538
GTATCAA5550.044.1354561
CATAGCG250.001701777737.59650480-81
GTACATG14100.036.749451
ACATGGG14250.034.965613
TACATGG14550.034.8907932
CATGGGG6600.034.185934
GCCGCGC550.002713857734.1786428
GGTTGAC702.1809147E-433.5683067
ATCAACG7750.032.14583
AACGCAG8000.031.7288156
GTATTAA600.004108673431.4040781
GTGCTAG902.5456387E-531.4040741
TCAACGC7950.031.33714
CAACGCA8050.030.947825
GGTCTAG650.006076968728.9883751
ATGGGGA3550.027.806165
ACGCAGA9200.027.584397
CCCTATA700.00872541826.9177761
GTGTAGA1406.2084473E-726.9177761
Produced by FastQC (version 0.11.2)