FastQCFastQC Report
Sun 14 Apr 2019
SRR6576307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326657
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5960.18245437875202428No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3350.10255405517102037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9150.042.1271481
CAAACGC500.001698870137.6061862
CAACGTA250.001703955137.58891754-55
ATTACTC500.001702708237.5889173
GTACATG19450.036.4944341
TACATGG19650.035.883772
ACATGGG19250.035.6362463
ATCAACG11150.034.133443
AACGCAG11200.033.9810526
TCAACGC11300.033.6803364
CAACGCA11700.032.930465
CTATTAC753.2672938E-431.3384881
GTATAAC600.004153314531.3384881
CGTCGGG600.004162663631.32409794
CATGGGT4550.030.9798774
CATGGGG7800.029.516944
ACGCAGA12900.029.502937
TATCAAC13150.029.3128052
CGCAGAG13100.029.0525028
CCTATAC650.00615666928.9145533
Produced by FastQC (version 0.11.2)