FastQCFastQC Report
Sun 14 Apr 2019
SRR6576301_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576301_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279902
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4030.14397896406599453No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2980.10646583447063615No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2900.10360769126337074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.10146408385792169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6400.039.788051
GTACATG14250.037.724971
ACATGGG14300.037.478863
TACATGG14100.037.3436242
GTCCTAC702.138693E-433.6830061
ATCAACG7950.031.9333293
CAACGCA8000.031.7337475
AACGCAG8050.031.536646
TCAACGC8050.031.536644
CCCCTAT600.0040886931.4374751
TTATAGT1052.0535717E-631.341974
ATGGGTA2450.030.702345
CATGGGA7300.030.2686184
TAAGCCT1252.3467146E-730.0882914
CATGGGT3800.029.6923924
ACACCGA650.006138052328.9310476
GTATAAC650.006138052328.9310473
TATCAAC9150.027.7453522
GTGTTAT856.6723715E-427.7389471
GTGTTAA1205.6741355E-627.5077921
Produced by FastQC (version 0.11.2)