FastQCFastQC Report
Sun 14 Apr 2019
SRR6576295_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576295_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377970
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5460.14445590919914278No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4610.12196735190623594No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3970.10503479112098843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA250.00523414556.3977789
GTATCAA8200.054.0045241
ATCAACG9600.045.5415043
CAACGCA9650.044.8183825
TCAACGC10000.043.249744
AACGCAG10150.042.610586
GTACATG16700.039.493691
TACATGG17050.038.0496252
ACATGGG17300.037.2280353
ACGCAGA11800.036.6426247
CGCAGAG11900.036.3347058
TATAGGG651.4098262E-436.161992
TATCAAC12250.036.0734332
GTATTAC550.00268367534.262061
GTAATAC902.585372E-531.3403933
GCAGAGT13900.030.7685729
CATGGGG6800.030.4186134
CATGGGA9900.029.9158294
TGAACGG650.006142250728.9295925
GGTATCA3650.028.3952671
Produced by FastQC (version 0.11.2)