FastQCFastQC Report
Sun 14 Apr 2019
SRR6576293_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576293_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218606
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2440.11161633258007556No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG250.00523114256.397945
GTATCAA4600.052.2023161
TACCGTC601.5995356E-646.9982837
TCAACGC5800.040.5157624
ATCAACG5850.040.169473
TATCAAC6250.038.35062
CAACGCA5850.037.7593045
AACGCAG6050.037.2878956
AGCCCGT906.1615174E-736.5542226
TACATGG9450.035.3108832
ATACCGT801.154657E-535.2487146
ACATGGG10400.032.9891853
GTACATG10100.032.632781
GTAATGC600.004154423331.332193
ATACGCT1201.6424565E-731.332198
TATACGC1201.6424565E-731.332197
ACGCAGA7050.031.3321887
CGCAGAG7050.031.3321888
CATGGGA5600.029.3739284
CCGTCGT804.7693925E-429.3739289
Produced by FastQC (version 0.11.2)