FastQCFastQC Report
Sun 14 Apr 2019
SRR6576293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218606
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2540.11619077243991473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA4950.054.121211
ATCAACG6650.040.992483
CAACGCA6800.040.0882385
TCAACGC6800.040.0882384
CTACGAC500.00169901437.6000024
AACGCAG7350.036.448986
CTAGTAT550.002712220234.1818163
TATCAAC7750.033.9612922
CGCAGAG8300.032.277118
ACGCAGA8550.031.3333327
GGTCTAA753.2669393E-431.3333321
GTACATG10400.030.730771
ACATGGG10700.029.8691583
TACATGG10700.029.4299052
CTAAGCT804.7683722E-429.3754
GCAGAGT9750.028.4410259
CTATCTG1057.3945856E-526.8571439
TAAGCTA700.00882066126.8571435
ATAGGAC1257.985123E-626.323
ATGGGTA2156.548362E-1126.2325575
Produced by FastQC (version 0.11.2)