FastQCFastQC Report
Sun 14 Apr 2019
SRR6576290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237172
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7750.32676707199838095No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4040.1703405123707689No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4020.16949724250754727No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3290.13871789249995783No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3270.1378746226367362No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3180.1340799082522389No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3000.12649047948324424No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2870.12100922537230363No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2730.11510633632975224No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2680.11299816167169817No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2630.1108899870136441No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2560.10793854249236841No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2530.10667363769753596No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2510.10583036783431433No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2500.10540873290270353No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2390.10077074865498456No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2390.10077074865498456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7800.037.3666881
ACATGGG15800.033.018843
GTACATG17100.031.614711
TACATGG17100.031.614712
TAAGGGA753.2678538E-431.3331934
TATAACT753.2678538E-431.3331934
CATGGGG6950.029.7552644
GGTACCT2700.029.5924598
ATCAACG9900.028.9594673
AACGCAG9800.028.7753816
CAACGCA10050.028.5272375
TCAACGC10100.028.3860114
TAATTGC1354.6087916E-727.8517254
TTAAACT1354.6087916E-727.8517254
ATAATTG1555.0480594E-827.29023
CATGGGA6800.026.9557614
GGCCAAT700.00881326626.8626861
TCTTAGA700.00882233726.8570233
TCTTACC700.00882233726.8570233
TATTGTA700.00882233726.8570235
Produced by FastQC (version 0.11.2)