FastQCFastQC Report
Sun 14 Apr 2019
SRR6576289_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576289_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309771
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5530.17851897046527918No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3620.11686051954508331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.11169541370883652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC704.6319165E-640.2760283
TACATGG16150.037.247832
ACATGGG16300.036.6108253
GTACATG16900.036.4820821
GTAATAG702.163781E-433.6176221
GTATCAA8050.033.3252941
CATGGGT3050.032.3528754
GGTACCT2050.032.1157658
CATGGGG7400.031.7491234
GCGTGAT300.004161122331.33085886-87
TAGTACT1052.0627995E-631.32584
TCTACAC1252.3587381E-730.072773
GGGTACC2200.029.90197
AACGCAG9000.029.7595126
ATACCGT650.006154548428.9161226
CAACGCA9350.028.6455175
ATGGGTA2500.028.193225
ATGGGGA3850.028.071175
ATCAACG9550.028.0456123
TGGGTAC2350.027.9932696
Produced by FastQC (version 0.11.2)