FastQCFastQC Report
Sun 14 Apr 2019
SRR6576289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309771
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5620.181424342498168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.1016880211511084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGT555.2840252E-542.7136968
GTATCAA7250.040.8547821
GTACATG17050.036.674791
TACATGG17100.035.4677772
ACATGGG16750.035.3496973
GTCTAGA851.7467351E-533.187361
TATAAGT851.7525375E-533.1712844
AACGCAG9350.032.1609046
ATCAACG9300.031.3284383
ATACTAC753.276161E-431.323386
TGTACCG753.276161E-431.323385
CTGTCGA600.004162801431.3233789
CAACGCA9500.030.6639395
CATGGGG7550.030.4985434
TCAACGC9650.030.192174
GTACTAC1102.9516614E-629.9189071
GTATATA1303.3088145E-728.932571
CTATAGA1154.1727126E-628.6180841
TATCAAC10500.028.2092552
ACGCAGA10800.027.4079577
Produced by FastQC (version 0.11.2)