FastQCFastQC Report
Sun 14 Apr 2019
SRR6576287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256637
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6040.23535187833398927No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3750.14612078538948006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.1379380214076692No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3250.12663801400421607No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3140.122351804299458No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3040.11845525002240519No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3030.1180655945946999No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2910.11338972946223654No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2780.1083242089020679No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2730.1063759317635415No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2700.10520696548042567No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2620.10208972205878342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT250.00523317956.3952031
CTATACC450.001013877541.766094
GTACATG20000.038.066761
TACATGG19750.037.8347552
ACATGGG19600.037.3976943
GTATAAC651.4111376E-436.1507721
CATGGGT3900.034.938944
GACCGTG550.002716820534.1722537
CCTATAC550.002716820534.1722533
CCTATTC1405.0749804E-1033.5620353
TGGGTAC2000.032.8907936
CTATTCC1457.403287E-1032.404724
GTATCAA9150.031.3306711
CATGGGC3600.031.3245664
GTTCTCG300.00416455631.32456626-27
GAGTAGC600.004160711531.3245666
GGGTACC2100.031.3245667
CATGGGG6150.031.3245644
TATTAGG1102.9585735E-629.906552
CATACCT953.7482052E-529.6759034
Produced by FastQC (version 0.11.2)