FastQCFastQC Report
Sun 14 Apr 2019
SRR6576285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319200
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7120.2230576441102757No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4550.1425438596491228No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3800.11904761904761905No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3700.11591478696741854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.11466165413533834No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3330.10432330827067669No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3270.10244360902255639No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3260.10213032581453635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA250.005235208756.3928574
GCAAACG555.2784333E-542.721861
GTACATG22350.039.1091421
TACATGG22250.038.868582
ACATGGG22050.038.788743
CATGGGG8200.035.5320854
ATGGGAG5050.032.5701335
GTATCAA10900.032.3353541
CAAACGC753.2697074E-431.3342742
TATAGGG600.004155910631.3342742
CATGGGA12500.029.3242864
AACGCCG856.8102696E-427.6435575
ATGGGGG3300.027.0571785
AACGCAG12600.026.853746
GTCGGAT350.00884534526.84953558-59
TCAACGC12900.026.2292374
CATGGGT4500.026.1078034
GGCGTGT909.5095986E-426.1078038
CAACGCA13150.025.7305835
ATCAACG13200.025.6331163
Produced by FastQC (version 0.11.2)