FastQCFastQC Report
Sun 14 Apr 2019
SRR6576277_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576277_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244944
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5270.215151218237638No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3010.12288523090992226No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2960.12084394800444183No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2920.1192109216800575No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2530.10328891501731009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.102880658436214No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2480.10124763211182965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG14400.039.818152
GTACATG14500.039.665011
ACATGGG14900.037.535693
CATGGGG5000.035.7188344
GTATCAA8000.034.1785621
TACTTAG600.004155311331.3323147
TTAACTA600.004155311331.3323144
CATGGGC2850.029.6832434
ATGGGGC1951.8189894E-1128.9221345
AACGCAG9100.028.9221326
ATCAACG9600.027.4157733
CAACGCA9800.027.3358445
TCAACGC9800.027.3358444
TATAGCC700.00882409926.8562682
ACGCAGA10550.025.8380227
CATGGGA8200.025.7918424
GTCTTAA1109.943999E-525.7142771
CGCAGAG10450.025.6355298
CATGGGT3500.025.5134564
TCTAGAT2451.2732926E-1124.9379632
Produced by FastQC (version 0.11.2)