FastQCFastQC Report
Sun 14 Apr 2019
SRR6576275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229785
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3200.13926061318188743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8150.040.9509471
AACGCAG9200.036.7801366
CAACGCA9300.035.373975
TCAACGC9400.034.997654
ATCAACG9150.034.9266173
TACATGG15750.034.919542
GTATAGC959.395917E-734.636791
GTACATG15900.033.998831
ACATGGG15900.033.991433
TAGACAG851.749509E-533.1742445
CTATACT851.749509E-533.1742444
ACGCAGA10400.031.6324927
GGTTACA1052.0534571E-631.338051
CCTATAA600.004151058431.3380492
CAATACG300.004159773231.3312338-39
GGGATCT600.00415548431.331237
CATGGGG4750.030.6716234
TATCAAC10600.030.5989462
CGCAGAG10800.030.4609158
GCCTATA650.006139545728.9274311
Produced by FastQC (version 0.11.2)