FastQCFastQC Report
Sun 14 Apr 2019
SRR6576274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323625
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10890.33650057937427574No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6340.19590575511780609No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5320.16438779451525687No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5320.16438779451525687No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4820.14893781382773272No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4760.14708381614522983No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4700.14522981846272692No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4120.12730784086519892No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3970.12267284665894167No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3910.12081884897643877No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3660.11309385863267671No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3640.11247585940517574No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3610.1115488605639243No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3550.1096948628814214No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3490.1078408651989185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.10536886828891463No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3400.10505986867516415No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3360.10382387022016223No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3260.1007338740826574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCC250.00523813556.3850759
GTACATG18250.042.4819031
CATGGGG7750.042.4403764
ACATGGG18300.042.1090743
TACATGG19100.041.0833632
CATGGGT5150.041.057094
ATGGGTA3750.040.0960545
GTATCAA8250.037.5900541
GGTACCT3500.036.2475478
ATGGGGA4950.035.1220175
GGGTACC3850.034.172777
TAGACGA600.00416199431.325049
TGGGTAC4400.030.9690746
TAATACC953.7508093E-529.6763554
AACGCAG10450.029.6763556
TAACGCC1303.3268225E-728.9154224
ATTGCAC650.006155680428.9154223
GTACCTG4400.028.8332759
CTGGTCG1005.321476E-528.1925379
GTATAGT1005.321476E-528.1925371
Produced by FastQC (version 0.11.2)