FastQCFastQC Report
Sun 14 Apr 2019
SRR6576273_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576273_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358486
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9870.27532455939701966No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6190.17267062033105895No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5780.16123363255468834No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5190.14477552819356962No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4710.13138588396757475No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4480.1249700127759522No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4400.12273840540495305No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4230.11799623974157987No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4150.11576463237058073No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4100.11436987776370625No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3900.1087908593362084No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3890.10851190841483349No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3750.10460659551558499No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3670.10237498814458584No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3610.10070128261633647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGC555.1829513E-542.8559951
GTATCAA8050.036.307811
GTACATG18850.034.2620621
GTATAGG851.715733E-533.276421
TACATGG19150.033.1309972
ACATGGG19450.032.619983
GTATTAC953.669026E-529.7736421
GTATAGA953.669026E-529.7736421
CATGGGG9050.028.561674
CAACGCA10950.027.039334
TATAGAG700.00883087126.8553892
AACGCAG11250.026.7360325
TCAACGC11250.026.3182813
ATCAACG11300.025.7859273
ATACAAT1859.995347E-925.4037466
GTATAAT1901.2845703E-824.8113671
CACTCTA3200.023.4984669
ATGGGGC3600.023.4984655
CATGGGT6050.023.3042644
ACGCAGA13150.022.873036
Produced by FastQC (version 0.11.2)