FastQCFastQC Report
Sun 14 Apr 2019
SRR6576273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358486
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10600.2956879766573869No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6110.1704390129600598No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5780.16123363255468834No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5500.15342300675619133No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4910.1369649023950726No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4570.12748057106832625No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4510.1258068655400769No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4400.12273840540495305No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4070.11353302499958157No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4000.11158036854995732No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3870.1079540065720837No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3800.10600135012245944No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3670.10237498814458584No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3640.10153813538046116No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3590.1001433807735867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8600.039.9116971
GTACATG19100.039.880211
TACATGG19600.038.8628582
ACATGGG19950.037.69393
ATCAACG10250.033.014343
CATGGGG9150.032.8740234
TCAACGC10500.032.2282834
CAACGCA10550.032.0755425
AACGCAG10550.032.0755426
CATGGGA7650.029.4899334
CATGGGT5600.029.3747394
TGTACCG804.774646E-429.3747395
TACTGTC650.006148864528.922827
ACGCAGA11800.028.6777117
GGTACCT3500.028.1997498
ATGGGTA4050.027.8516035
CGCAGAG12200.027.737468
GGTATCA4750.027.7166161
CATGGGC3900.026.5125834
TGGGTAC3950.026.1769836
Produced by FastQC (version 0.11.2)