FastQCFastQC Report
Sun 14 Apr 2019
SRR6576272_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576272_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212153
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4230.19938440653679185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2160.10181331397623412No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2150.10134195604115898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC405.631386E-447.0587651
CATGGGT3200.039.649694
TACATGG15200.038.0355642
GTACATG15150.037.895511
ACATGGG14800.037.466773
ATGGGTA1850.035.561685
GTATTGG702.163083E-433.6134031
TGGGTAC1850.033.021566
GGGTACC1850.033.021567
CATGGGG4950.032.277494
GTATCAA7150.032.2500651
GGTTAGC600.004147188731.3429228
GGGTTAG600.004156773431.3281487
AAGGCGT1052.0575462E-631.3281486
AACGCAG7550.030.4982626
AGGCGTG1102.9572457E-629.904147
GGTACCT2051.8189894E-1229.8148
CGGTAGT404.78049E-429.37013824-25
CAACGCA7900.029.1470745
TTAGGAC650.00614798428.9182913
Produced by FastQC (version 0.11.2)