FastQCFastQC Report
Sun 14 Apr 2019
SRR6576269_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576269_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656371
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9670.14732521698856288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.12462464063768813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13200.054.974031
CAACGCA16550.042.5968785
TCAACGC16650.042.3410384
ATCAACG17050.041.3477023
AACGCAG17650.040.4746746
GTACATG29100.035.461891
TATCAAC20300.035.433352
GGTATCA5750.035.2380141
TACATGG28750.035.157462
ACGCAGA20350.034.642677
TCTATAC1800.033.94343
ACATGGG29750.033.807363
CGCAGAG21000.033.5703968
CATGGGT7650.028.8749284
GCAGAGT24900.028.123639
CATGGGG15000.025.6925414
ATGGGTA5400.025.2399645
CATGGGA13450.025.1590754
GTATAGG1951.7065759E-824.164411
AGAGTAC22350.023.13127910-11
Produced by FastQC (version 0.11.2)