FastQCFastQC Report
Sun 14 Apr 2019
SRR6576268_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576268_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201937
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3570.17678781005957303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2740.13568588223059666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.11637292819047525No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2250.11142088869300822No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2070.10250721759756756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG16450.037.4231452
GTACATG16550.036.9496841
ACATGGG16350.035.9275173
CATGGGT3150.034.312494
ATGGGTA1850.033.022245
GGTACCT1309.054929E-932.5579348
ATGGGTG1457.366907E-1032.40915
GTATCAA9050.032.226151
CATGGGA8750.031.6868364
AACGCAG9800.029.7303876
AACGAAC804.750414E-429.3925894
AAAGGCG804.7711423E-429.3707435
ACTATAC650.00614667428.9188863
AATATAG650.00614667428.9188865
TCATAGC650.00614667428.9188863
GTATGAT1005.271619E-528.2238731
ACAACAG856.802267E-427.6430515
CATGGGC3450.027.2424284
GGGTACC1606.866685E-826.4336687
AGGCGTG909.498465E-426.1073277
Produced by FastQC (version 0.11.2)