FastQCFastQC Report
Sun 14 Apr 2019
SRR6576266_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576266_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156344
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3800.24305377884664586No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.2040372511896843No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2190.14007573044056695No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2130.13623803919561991No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1920.12280611983830528No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1880.12024765900834058No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1790.11449112214092003No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1760.1125722765184465No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1760.1125722765184465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.10937420048099064No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1700.10873458527349945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG1600.038.1821755
GTATCAA7550.036.758631
CATGGGT2450.036.4438934
GCTCTAG702.1659373E-433.5989881
CATGGGG3950.033.3118064
ACATGGG12900.032.421833
GTACATG13550.032.2847861
TACATGG13500.032.0354082
ACTGTTC902.5709065E-531.3490188
CATAGGG600.00414642531.338992
ATCAACG9200.030.6478983
AACGCAG8800.030.4389346
TCAACGC9450.029.8371074
GTACTGT953.7353548E-529.6800676
CAACGCA9450.029.339825
GTATAGA650.006113558528.9468211
ATGGGTA1154.170508E-628.6047045
CATGGGC1503.6598067E-828.1960644
TACTGTA856.795126E-427.6432027
TGTGTAC856.795126E-427.6432023
Produced by FastQC (version 0.11.2)