FastQCFastQC Report
Sun 14 Apr 2019
SRR6576266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156344
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3820.24433300926162818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.20019955994473723No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2110.13495880878063757No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2020.129202271913217No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1890.12088727421583176No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1770.11321189172593768No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1750.11193266131095533No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1620.10361766361357007No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1590.10169881799109656No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1570.1004195875761142No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAG200.002156762270.522321
CTTAGAC250.00523429956.3817633
TACATGG13850.037.0008852
ACATGGG13650.036.830583
GTACATG14000.036.6044461
GTATCAA7250.035.6664621
CATGGGC1651.8189894E-1234.170774
ATGGGAG2650.033.687225
ATGGGAC1405.0567905E-1033.5605745
CATGGGA8200.032.6601684
CATGGGT2750.032.4622274
TTCTAGG753.2566473E-431.3432562
TGGGTAC1751.6916601E-1029.5333066
TATACGC1452.6375346E-829.1629837
AACGCAG8700.029.1629816
GTATTAT650.006128635728.9322361
GTAACTT650.00614783928.9137253
ATCAACG9050.028.55433
TCAACGC9050.028.55434
ATATACG1652.932211E-928.475646
Produced by FastQC (version 0.11.2)