FastQCFastQC Report
Sun 14 Apr 2019
SRR6576265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169006
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3840.2272108682531981No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.16922476125107985No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2160.12780611339242393No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2140.1266227234536052No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2140.1266227234536052No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2080.12307255363714897No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1940.1147888240654178No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1770.1047300095854585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7400.045.0945931
TACATGG15600.037.3589742
ACATGGG15450.037.113273
GTACATG15900.036.6540871
TTACACT906.14873E-736.5555534
CATGGGT3400.035.9411774
GTCCTAT801.1528196E-535.251
AACGCAG9400.034.56
ATGGGAG2950.033.4576265
ATCAACG9800.033.0918353
CATGGGA8150.032.8711664
TCAACGC10050.032.2686544
CAACGCA10100.032.108915
TATATCT600.004151037431.3333344
GGTCGGA753.263825E-431.3333327
TATCAAC10600.031.0377372
CATGGGG4550.029.9560434
TACGCTA1602.1063897E-929.3759
ACGCAGA11100.029.2162157
CGCAGAG11150.029.0852018
Produced by FastQC (version 0.11.2)