FastQCFastQC Report
Sun 14 Apr 2019
SRR6576262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648761
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17340.267278705100954No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9680.1492074893527817No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9120.14057565112576126No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8740.13471833232885452No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8020.1236202546083997No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6930.1068189980593778No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6780.10450689853428305No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6770.1043527585659434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT405.678046E-446.9946821
GTATCAA14100.043.328431
GTACATG36300.040.003741
TACATGG36700.039.442722
ACATGGG35600.039.3352133
CATGGGG16700.035.454334
CATGGGT9550.034.4437074
TCAACGC18250.032.958114
CAACGCA18350.032.266345
ATGGGGA8900.032.2073555
ATCAACG18700.032.1653
AACGCAG18800.031.9939146
ATGGGTA7150.028.9175765
GTATATG2300.028.605461
ACGCAGA21800.027.591087
TACTATA1205.8396745E-627.4156782
CGCAGAG22200.027.0939458
TACTAGC700.0088347926.8561732
GGTACCT7000.026.8520348
TATCAAC23250.026.2786242
Produced by FastQC (version 0.11.2)