FastQCFastQC Report
Sun 14 Apr 2019
SRR6576261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706040
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16810.2380884935697694No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9960.1410684947028497No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9470.13412837799558097No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8640.12237267010367685No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7680.10877570675882385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15850.046.2670061
ACATGGG37950.035.666833
GTACATG39100.035.4667431
TACATGG39350.035.002492
TCAACGC20650.034.8221134
AACGCAG21150.034.2211156
ATCAACG21300.033.980123
CAACGCA21250.033.83895
TAGTATC753.2748937E-431.332324
ACGCAGA23950.030.024087
CGCAGAG24500.029.3500698
CATGGGG18950.029.0175274
GTATAGA1303.3232936E-728.9282861
CATGGGT10300.027.3777544
TATCAAC27200.026.7879122
CATGGGA15500.026.3797914
ATGGGTA7100.025.8160655
ATGGGAG7500.025.69255
GGTACCT7450.025.2340818
GCAGAGT29350.024.500069
Produced by FastQC (version 0.11.2)