FastQCFastQC Report
Sun 14 Apr 2019
SRR6576260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270698
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6760.24972478555438166No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3660.13520602294808237No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3190.11784350087551441No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.1134105165165609No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2870.10602220925163837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG708.582538E-846.9955671
GTATCAA9350.044.231121
AAACGCA652.782339E-643.372513
CCGCTAA450.001013972241.766124
CCCGCTA450.001013972241.766123
AACGCAG10050.038.8050886
CGTAATA250.001703565837.58950852-53
TACATGG15800.037.4774782
GTACATG16100.036.7791371
ATCAACG10750.036.7153323
ACATGGG16450.036.275593
CAAACGC651.4114195E-436.1504332
TCAACGC11050.035.7185364
CAACGCA11200.035.240165
CCAATAC550.002717072634.172283
ACGCAGA11600.033.6199267
CGCAGAG11800.033.0500958
CCTTATG600.00415733331.3303762
CATGGGT3900.031.3245894
CATGGGG7950.030.1425294
Produced by FastQC (version 0.11.2)