FastQCFastQC Report
Sun 14 Apr 2019
SRR6576259_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576259_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297331
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6580.2213021851068338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.13654815676804638No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3760.12645839148961932No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3700.12444043843393389No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3690.124104112924653No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3130.10526988440492245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9550.042.9880451
GTAACAT450.001012162341.782643
GTACATG18500.037.495351
ACATGGG18250.037.346813
TCAACGC11100.036.842124
ATCAACG11150.036.676913
CAACGCA11300.036.6060265
TACATGG18700.036.448092
AACGCAG11600.036.064546
CATGGGT4400.034.1857954
CATGGGG9000.031.8592624
TGCACTA600.00415370131.3369775
ACGCAGA13250.031.2134767
TATCAAC13450.030.7545052
CGCAGAG13450.030.7493328
GGTACCT2850.029.682678
CCCTTAA804.6621443E-429.492521
GCCTATG650.006028155329.0387841
GTAAGAG650.006143439628.926443
ATGGGTA2950.028.6813035
Produced by FastQC (version 0.11.2)