FastQCFastQC Report
Sun 14 Apr 2019
SRR6576259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297331
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6760.22735604427389003No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3760.12645839148961932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA200.002161674270.4977343
GTATCAA8300.037.9640771
GTACATG18400.036.2949871
TACATGG18450.036.196632
CATGGGT5050.035.3651964
ACATGGG18750.034.8415453
TCAACGC10050.032.7352644
AACGCAG9750.032.2963946
CAACGCA9900.032.2817885
ATCAACG10050.031.799973
GTATTAA1052.048293E-631.3534181
ATGGGTA3900.030.1272355
GTCTATG804.754582E-429.393832
ATGGGAG3700.029.2152755
TGGGTAC3400.029.0284776
GTACTAG650.00612771428.9416161
GTATAGA650.00612771428.9416161
CATGGGG8700.028.6312624
ACTAGGC1005.31162E-528.1990913
TAGGGGT700.00882706826.8562774
Produced by FastQC (version 0.11.2)