FastQCFastQC Report
Sun 14 Apr 2019
SRR6576258_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576258_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547157
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.1652176614755911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13500.043.9898761
ACGTTAT550.00267867334.2778241
TAGACCC902.5920275E-531.332334
TCAACGC18600.031.3323294
ATCAACG18600.031.0796493
CAACGCA18950.030.5056175
AACGCAG19000.030.4253396
TATCAAC19550.029.8152332
GTATTAG1354.5150955E-727.9300821
GTACATG25000.027.5250951
ACGCAGA22350.026.0752267
TACATGG25750.025.9223522
ACATGGG25650.025.6521973
CATGGGG13550.024.973374
CGCAGAG23200.024.9173038
TATACTG2052.9800503E-822.9260925
AGAGTAC23700.022.40856410-11
GCAGAGT26800.021.7455719
AGTACTT14300.021.19862212-13
GTACTAC1353.996875E-420.9475611
Produced by FastQC (version 0.11.2)