FastQCFastQC Report
Sun 14 Apr 2019
SRR6576257_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576257_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316346
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6600.2086323203075114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.1631125413313271No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3930.12423106345583633No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3820.12075385811737781No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3340.10558059845864971No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3340.10558059845864971No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3320.1049483793062027No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTC200.002163618370.482815
CGTCTCA250.00521795856.439788
GTATCAA11600.043.033041
GTACATG23750.041.83821
TACATGG24000.041.121472
ACATGGG24900.039.440183
CATGGGG8850.033.980414
CATGGGA13700.032.5832944
AACGCAG15300.032.564456
TCAACGC15300.032.5541534
CAACGCA15550.032.0307775
ATCAACG15650.031.8261013
GATACTA600.004154992331.3356026
CATGGGT5000.029.1328934
ACGCAGA17300.028.799777
ATGGGAG5400.028.7152185
TTACTAC1005.3203476E-528.1931253
TATCAAC17800.027.9863642
ATGGGAT3700.027.9391335
CGCAGAG17650.027.7135838
Produced by FastQC (version 0.11.2)