FastQCFastQC Report
Sun 14 Apr 2019
SRR6576257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316346
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6960.22001226505155747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.14509429548658748No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4130.13055325498030637No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3680.11632832405024877No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3410.10779336549221422No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3200.10115506439152067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10050.045.824451
ACATGGG24650.041.3629383
GTACATG25000.041.354231
TACATGG25000.041.354232
TAGTACT853.952755E-738.6943634
CATGGGT5200.037.950244
CATGGGG9650.036.517624
AACGCAG12550.036.315886
CTAGTAC959.4438656E-734.621273
ATCAACG13000.034.3359343
GTATAAG1107.6757715E-834.1770441
CATGGGA11100.033.8637924
CAACGCA13400.033.3109785
TCAACGC13400.033.3109784
TATAAGG851.7524539E-533.171842
TCTAGAC600.00416252531.3240073
GTAATGC600.00416252531.3240073
CTCGATA300.004165644831.32400764-65
CGATAAT300.004165644831.32400766-67
TATCAAC14700.031.0092752
Produced by FastQC (version 0.11.2)