FastQCFastQC Report
Sun 14 Apr 2019
SRR6576254_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576254_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407457
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7070.1735152420991663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.13081134941846626No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4280.10504175900769898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11400.047.885081
GTACATG23850.040.2521321
TACATGG23800.040.0847852
ATCAACG13750.038.9639133
AACGCAG14250.038.586156
ACATGGG24150.038.530843
TCAACGC14050.038.1319474
CAACGCA14400.037.857855
CATGGGA13500.035.5080534
CGCAGAG16300.033.7332888
ACGCAGA16500.033.32447
TATCAAC16300.033.156652
GGTATCA5000.030.1180631
GCAGAGT18750.029.5761189
ATGGGAG6050.029.5181165
CATGGGT5600.029.372474
ATGGGTA3250.028.9205865
CATGGGG8150.028.2552344
CTCGTAC551.01772705E-425.63730272-73
ACCGACC950.001303795724.734718
Produced by FastQC (version 0.11.2)