FastQCFastQC Report
Sun 14 Apr 2019
SRR6576253_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576253_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488162
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9310.19071537727229895No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6860.14052712009537816No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5430.11123356590639992No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5200.10652201523264818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13050.048.712911
AACGCAG16500.038.729396
ATCAACG16300.038.628063
TCAACGC16550.037.760644
TACATGG27200.037.4866372
CAACGCA16750.037.3097655
ACATGGG28050.036.5181963
GTACATG28250.036.3378221
TATCAAC19150.034.596812
CGCAGAG19650.032.5441938
ACGCAGA19650.032.5208637
CATGGGA15750.030.728574
CATGGGT7450.030.274064
TTAGTAC650.00615851328.9156063
ATGGGAG7050.028.6592645
GCAGAGT23700.027.181259
GGGTACC4150.027.1737027
CATGGGC5250.026.8502064
GGTACCT4550.024.802598
GTATAGG1901.3023964E-824.7837641
Produced by FastQC (version 0.11.2)