FastQCFastQC Report
Sun 14 Apr 2019
SRR6576253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488162
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8600.1761710251924566No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5780.11840331693167433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.10590746514476752No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4940.10119591447101577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12600.047.013891
ATCAACG16050.036.5926743
AACGCAG16250.036.4314426
CAACGCA16400.036.098235
TCAACGC16350.035.921254
TACATGG26200.033.7351532
TTAGTAC702.1872667E-433.5607073
GTACATG26700.033.279491
ACATGGG26600.033.207443
TATCAAC19100.031.0143992
ACGCAGA19150.030.9144087
CGCAGAG20000.029.6005488
CATGGGG10700.027.6640624
CATGGGT5600.026.8485664
CATGGGA15000.025.6851314
GTATCAT1859.975338E-925.4129121
GTAATAG1301.0888503E-525.2996123
GCAGAGT23400.025.299619
ATGGGAG6400.024.2266395
TAATAGA2402.6193447E-1023.4924984
Produced by FastQC (version 0.11.2)