FastQCFastQC Report
Sun 14 Apr 2019
SRR6576252_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576252_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541391
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9200.16993263648638415No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.1207999394153209No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5930.1095326667787237No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5440.10048190679194889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12200.049.4289131
ATCAACG15100.037.9725383
AACGCAG14750.037.2804076
CAACGCA15150.037.2267725
TACATGG27950.036.4892272
TCAACGC15500.036.3861664
GTACATG28450.036.099831
ACATGGG28100.035.9599343
TATCAAC18400.032.183962
ATGGGAG7050.031.9991825
CATGGGT6850.030.8751224
CATGGGA15250.030.2025074
ACGCAGA18400.030.1405357
CGCAGAG18850.029.919668
CCTATAC1602.1282176E-929.374253
GTATATA3050.027.8037641
CATGGGG9700.026.6488024
GGGTACC3800.025.973027
GCAGAGT22200.025.4047579
ATGGGTA4500.025.0660275
Produced by FastQC (version 0.11.2)